Experiment summary for ENCSR283GOW

doi:10.17989/ENCSR283GOW

Summary

Status
released
Assay
small RNA-seq
Biosample summary
Drosophila melanogaster CME-W1-Cl.8+ cytosolic fraction
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq on rRNA-depleted total RNA in Drosophila cell line CME-W1-Cl.8+ (cytosol fraction), < 200 nucleotides in size that was CIP and TAP-treated prior to cloning.
Treatments
Antarctic Phosphatase for 30 minutes, Tobacco Acid Pyrophosphatase for 1 hour
Nucleic acid type
RNA
Depleted in
rRNA
Size range
<200
Extraction method
Trizol
Strand specificity
Strand-specific

Attribution

modENCODE Project
Lab
Thomas Gingeras, CSHL
Award
U01HG004271 (Susan Celniker, LBNL)
Project
modENCODE
Aliases
modencode:4233, susan-celniker:E-BS603C-BS604C_CIPTAP
Date submitted
March 31, 2014
Date released
March 31, 2014

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
21Drosophila melanogaster CME-W1-Cl.8+ cell line cytosolic fractionENCBS648NZIENCLB798ZVV
11Drosophila melanogaster CME-W1-Cl.8+ cell line cytosolic fractionENCBS371JYEENCLB715WAU