Experiment summary for ENCSR272KZJ
doi:10.17989/ENCSR272KZJ
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- lsy-12
- Biosample summary
- Caenorhabditis elegans strain OP568 (unc-119(tm4063) III; wgIs568) whole organism L3 larva 43 hours post synchronization at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans lsy-12
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing LSY-12-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation methods
- sonication (generic microtip)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:LSY-12-OP568-L3
- Date submitted
- October 4, 2016
- Date released
- October 11, 2016
- Submitter comment
- ENCFF306PVA, ENCFF608AUU, ENCFF464CIR, ENCFF857JRK were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 4 | 1 | Caenorhabditis elegans strain OP568 (unc-119(tm4063) III; wgIs568) mixed sex L3 larva 43 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans lsy-12 | ENCBS897PYA | ENCGM605SPT | ENCAB728YTO | ENCLB421EPS |
| 3 | 1 | Caenorhabditis elegans strain OP568 (unc-119(tm4063) III; wgIs568) mixed sex L3 larva 43 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans lsy-12 | ENCBS931EWW | ENCGM605SPT | ENCAB728YTO | ENCLB290FCX |