Experiment summary for ENCSR272KZJ

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
lsy-12
Biosample summary
Caenorhabditis elegans strain OP568 (unc-119(tm4063) III; wgIs568) whole organism L3 larva 43 hours post synchronization at L1 larva starved after bleaching stage genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing LSY-12-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
SPRI beads
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:LSY-12-OP568-L3
Date submitted
October 4, 2016
Date released
October 11, 2016
Submitter comment
ENCFF306PVA, ENCFF608AUU, ENCFF464CIR, ENCFF857JRK were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
41strain OP568 (unc-119(tm4063) III; wgIs568) mixed sex L3 larva 43 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting lsy-12ENCBS897PYAENCGM605SPTENCAB728YTOENCLB421EPS
31strain OP568 (unc-119(tm4063) III; wgIs568) mixed sex L3 larva 43 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting lsy-12ENCBS931EWWENCGM605SPTENCAB728YTOENCLB290FCX