1. Experiments
  2. CRISPR genome editing followed by RNA-seq
  3. Homo sapiens
  4. HepG2

Experiment summary for ENCSR268XHM

doi:10.17989/ENCSR268XHM

Summary

Status
released
Assay
CRISPR genome editing followed by RNA-seq (CRISPR RNA-seq)
Target
IGF2BP1
Biosample summary
Homo sapiens HepG2 genetically modified (deletion) using CRISPR targeting H. sapiens IGF2BP1
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq on HepG2 cells treated with a CRISPR gRNA against IGF2BP1. (IGF2BP1_BGHcLV03)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
10
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR358OMW
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
Aliases
brenton-graveley:IGF2BP1_BGHcLV03
Date submitted
March 9, 2016
Date released
March 17, 2016
Gene Silencing Series
ENCSR062UWG 
Tags
Visit collection page for ENCORESearch for all Experiment with internal tag ENCYCLOPEDIAv1Search for all Experiment with internal tag ENCYCLOPEDIAv2

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
21Homo sapiens HepG2 cell line genetically modified (deletion) using CRISPR targeting H. sapiens IGF2BP1ENCBS038ZQBENCGM078TECENCLB697TUE
11Homo sapiens HepG2 cell line genetically modified (deletion) using CRISPR targeting H. sapiens IGF2BP1ENCBS060DQWENCGM078TECENCLB072UCR

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