Experiment summary for ENCSR268AXN
doi:10.17989/ENCSR268AXN
Summary
- Status
- released
- Assay
- single-cell RNA sequencing assay (scRNA-seq)
- Control type
- control
- Biosample summary
- Mus musculus strain B6NCrl pyramidal neuron male adult (8 months)
- Biosample Type
- primary cell
- Replication type
- isogenic
- Description
- RNA-seq on a 50 cell pool of layer V pyramidal neurons from an 8 month old male wild type C57Bl5 mouse
- Nucleic acid type
- RNA
- Library starting quantity
- 50cells
- Size range
- >200
- Fragmentation methods
- chemical (Nextera tagmentation)
- Size selection method
- SPRI beads
- Strand specificity
- unstranded
- Spike-ins datasets
- ENCSR013YHQ, ENCSR133ALU
- Platform
- Illumina HiSeq 2500
Attribution
ENCODE3 project
- Lab
- Barbara Wold, Caltech
- Award
- U54HG006998 (Richard Myers, HAIB)
- Project
- ENCODE
- External resources
- Date submitted
- December 16, 2014
- Date released
- July 15, 2015
- Submitter comment
- Estimates of technical measurement error from low input mass RNA-seq libraries.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 2 | 1 | Mus musculus strain B6NCrl male adult (8 months) pyramidal neuron primary cell | ENCBS880BCZ | ENCLB730HDZ |
| 1 | 1 | Mus musculus strain B6NCrl male adult (8 months) pyramidal neuron primary cell | ENCBS599JIO | ENCLB893OOW |