Experiment summary for ENCSR265OMO
doi:10.17989/ENCSR265OMO
Summary
- Status
- released
- Assay
- whole-genome shotgun bisulfite sequencing (WGBS)
- Biosample summary
- Mus musculus strain B6NCrl heart tissue embryo (15.5 days)
- Biosample Type
- tissue
- Replication type
- isogenic
- Description
- Whole genome bisulfite-seq on embryonic 15.5 day mouse heart
- Nucleic acid type
- DNA
- Size range
- 180-500
- Lysis method
- QIAGEN DNeasy Blood & Tissue Kit
- Fragmentation methods
- shearing (Covaris S2)
- Size selection method
- Sera-mag SpeedBeads
- Platform
- Illumina HiSeq 2500
Attribution
ENCODE3 project
- Lab
- Joe Ecker, Salk
- Award
- U54HG006997 (Bing Ren, UCSD)
- Project
- ENCODE
- External resources
- Aliases
- joe-ecker:E15_5_HT
- Date submitted
- April 28, 2015
- Date released
- June 23, 2015
- Organism Development Series
- ENCSR781CPK
- Reference Epigenome
- ENCSR520JSM
- Submitter comment
- Fastq files ENCFF056IAF, ENCFF906CFP, ENCFF817MUC, and ENCFF072FHV contain reads of mixed lengths. Read lengths followed by respective number of reads: ENCFF056IAF (117, 138107905), (125, 196941499), (145, 192682496); ENCFF906CFP (117, 143659805), (125, 198660436), (145, 196855711); ENCFF817MUC (117, 183078032), (125, 198956732), (130, 165703094); ENCFF072FHV (117, 192971599), (130, 187667841), (132, 196068442).
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
---|---|---|---|---|
1 | 1 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) heart tissue | ENCBS595EMK | ENCLB857VNP |
2 | 2 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) heart tissue | ENCBS909LOF | ENCLB494KTO |
2 | 1 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) heart tissue | ENCBS909LOF | ENCLB154WOQ |
1 | 2 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) heart tissue | ENCBS595EMK | ENCLB466JGR |