Experiment summary for ENCSR255SDF

  • Status: released

Summary

Assay
RNA-seq (total RNA-seq)
Biosample summary
Mus musculus C57BL/6 midbrain postnatal (0 days)
Biosample Type
tissue
Replication type
isogenic
Description
The libraries contained in this experiment come from independent isolates of midbrain tissue. They are stranded PE101 Illumina Hi-Seq libraries from rRNA-depleted Total RNA > 200 nucleotides in size.
Nucleic acid type
RNA
Depleted in
rRNA
Size range
>200
Lysis method
see document
Extraction method
see document
Fragmentation method
see document
Size selection method
see document
Strand specificity
Strand-specific
Spike-ins datasets
ENCSR156CIL
Platform
HiSeq 2000

Attribution

ENCODE3 project
Lab
Thomas Gingeras, CSHL
Award
U54HG007004 (Thomas Gingeras, CSHL)
Project
ENCODE
External resources
Date released
2014-09-03
Tags
ENCYCLOPEDIAv4 tag

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleLibrary
11strain B6NTac;B6NCrl/Lap midbrain tissue mixed sex postnatal (0 days)ENCBS039AAAENCLB355PVM
21strain B6NTac;B6NCrl/Lap midbrain tissue mixed sex postnatal (0 days)ENCBS555AAAENCLB536RCP

Documents

excision protocol
Description: Specific excision protocol for embryonic and postnatal mouse biosamples
Submitted by
Axel Visel
Lab
Len Pennacchio, LBNL
general protocol
Description: This document describes the construction of library:thomas-gingeras:LID292124
Submitted by
Carrie Davis
Lab
Thomas Gingeras, CSHL
general protocol
Description: This document describes the construction of library:thomas-gingeras:LID292125
Submitted by
Carrie Davis
Lab
Thomas Gingeras, CSHL
pipeline protocol
Description excerpt: Long RNA-seq pipeline: Includes overview, references, and spike-ins information about the pipeline
Description
Long RNA-seq pipeline: Includes overview, references, and spike-ins information about the pipeline
Submitted by
Kathrina Onate
Lab
J. Michael Cherry, Stanford