Experiment summary for ENCSR250STJ

doi:10.17989/ENCSR250STJ

Summary

Status
released
Assay
small RNA-seq
Biosample summary
Homo sapiens smooth muscle cell originated from H9
Biosample Type
in vitro differentiated cells
Replication type
isogenic
Description
The libraries contained in this experiment come from H9-derived embryonic stem cells that were differentiated down a smooth muscle pathway. They are stranded SE101 length Hi-Seq reads from rRNA-depleted Total RNA <200 nucleotides in size that was pretreated with Tobacco Acid Pyrophosphatase prior to cloning.
Treatments
Tobacco Acid Pyrophosphatase for 1 hour
Nucleic acid type
RNA
Depleted in
rRNA
Size range
<200
Fragmentation methods
see document
Strand specificity
Strand-specific (forward)

Attribution

ENCODE3 project
Lab
Thomas Gingeras, CSHL
Award
U54HG007004 (Thomas Gingeras, CSHL)
Project
ENCODE
External resources
Date submitted
December 13, 2014
Date released
December 17, 2014
Reference Epigenome
ENCSR116JEF 
Tags
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Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
11Homo sapiens female embryo (5 days) smooth muscle in vitro differentiated cells originated from H9ENCBS780PCJENCLB875QBG
21Homo sapiens female embryo (5 days) smooth muscle in vitro differentiated cells originated from H9ENCBS899TTJENCLB795WKJ