Experiment summary for ENCSR240NHL

doi:10.17989/ENCSR240NHL

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
ac
Biosample summary
Drosophila melanogaster strain ac-GFP whole organism embryo (0-8 hours) genetically modified (insertion) using site-specific recombination targeting D. melanogaster ac
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing ac-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:ac-GFP
Date submitted
December 21, 2016
Date released
January 10, 2017
Submitter comment
ENCFF931VLQ, ENCFF407JJC, ENCFF235MMB, ENCFF541WSZ were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
31Drosophila melanogaster strain ac-GFP mixed sex embryo (0-8 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster acENCBS183GUGENCGM377WKQENCAB728YTOENCLB644GQN
11Drosophila melanogaster strain ac-GFP mixed sex embryo (0-8 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster acENCBS854AAQENCGM377WKQENCAB728YTOENCLB851WYC
21Drosophila melanogaster strain ac-GFP mixed sex embryo (0-8 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster acENCBS385CQFENCGM377WKQENCAB728YTOENCLB972EIQ