Experiment summary for ENCSR239IAH

doi:10.17989/ENCSR239IAH

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
bed-3
Biosample summary
Caenorhabditis elegans strain OP651 (unc-119(tm4063); wgIs651 [bed-3::TY1::EGFP::3xFLAG + unc-119(+)]) whole organism mixed stage (embryo) (4 hours) using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans bed-3
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic worms expressing bed-3-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:BED-3_OP651_EM
Date submitted
February 26, 2018
Date released
March 1, 2018

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11Caenorhabditis elegans strain OP651 (unc-119(tm4063); wgIs651 [bed-3::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex mixed stage (embryo) (4 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans bed-3ENCBS597ODYENCGM628FPGENCAB728YTOENCLB703YQT
31Caenorhabditis elegans strain OP651 (unc-119(tm4063); wgIs651 [bed-3::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex mixed stage (embryo) (4 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans bed-3ENCBS740YVOENCGM628FPGENCAB728YTOENCLB743KRL
41Caenorhabditis elegans strain OP651 (unc-119(tm4063); wgIs651 [bed-3::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex mixed stage (embryo) (4 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans bed-3ENCBS741QFFENCGM628FPGENCAB728YTOENCLB848SUG