Experiment summary for ENCSR227RPE
doi:10.17989/ENCSR227RPE
Summary
- Status
- released
- Assay
- whole-genome shotgun bisulfite sequencing (WGBS)
- Biosample summary
- Mus musculus strain B6NCrl hindbrain tissue embryo (15.5 days)
- Biosample Type
- tissue
- Replication type
- isogenic
- Description
- Whole genome bisulfite-seq on embryonic 15.5 day mouse hindbrain
- Nucleic acid type
- DNA
- Size range
- 180-500
- Lysis method
- QIAGEN DNeasy Blood & Tissue Kit
- Fragmentation methods
- shearing (Covaris S2)
- Size selection method
- Sera-mag SpeedBeads
- Platform
- Illumina HiSeq 2500
Attribution
ENCODE3 project
- Lab
- Joe Ecker, Salk
- Award
- U54HG006997 (Bing Ren, UCSD)
- Project
- ENCODE
- External resources
- Aliases
- joe-ecker:E15_5_HB
- Date submitted
- April 28, 2015
- Date released
- June 23, 2015
- Organism Development Series
- ENCSR839HKP
- Reference Epigenome
- ENCSR356VGB
- Submitter comment
- Fastq files ENCFF812DAL and ENCFF644JSE contain reads of mixed lengths. Read lengths followed by respective number of reads: ENCFF812DAL (108, 218287664), (130, 392620409); ENCFF644JSE (108, 219952521), (130, 417655336).
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 2 | 1 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) hindbrain tissue | ENCBS948UKU | ENCLB912YBJ |
| 1 | 2 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) hindbrain tissue | ENCBS958AHU | ENCLB261WOE |
| 1 | 1 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) hindbrain tissue | ENCBS958AHU | ENCLB395AEQ |
| 2 | 2 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) hindbrain tissue | ENCBS948UKU | ENCLB750FMV |