Experiment summary for ENCSR225LQP

Summary

Status
revoked
Assay
ChIP-seq (TF ChIP-seq)
Target
ztf-16
Biosample summary
Caenorhabditis elegans strain OP494 (unc-119(tm4063) III; wgIs494) whole organism hermaphrodite late embryo 5 hours post synchronization using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans ztf-16
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing ZTF-16-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:ZTF-16-OP494-LE
Date released
May 24, 2016
Submitter comment
ENCFF397HQQ, ENCFF485MPP were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21Caenorhabditis elegans strain OP494 (unc-119(tm4063) III; wgIs494) hermaphrodite late embryo 5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans ztf-16ENCBS066XFJENCGM642POYENCAB728YTOENCLB461IJQ
11Caenorhabditis elegans strain OP494 (unc-119(tm4063) III; wgIs494) hermaphrodite late embryo 5 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans ztf-16ENCBS658NLCENCGM642POYENCAB728YTOENCLB501JWO