Experiment summary for ENCSR224PTG

doi:10.17989/ENCSR224PTG

Summary

Status
released
Assay
polyA plus RNA-seq
Biosample summary
Homo sapiens A549 treated with 100 nM dexamethasone (agonist) for 10 hours
Biosample Type
cell line
Replication type
isogenic
Description
mRNA-seq on A549 cell line treated with 100 nM dexamethasone for 10 hours.
Treatments
100 nM dexamethasone (CHEBI:41879) for 10 hours
Nucleic acid type
polyadenylated mRNA
Library starting quantity
1µg
Size range
>200
Extraction method
RNeasy Plus Mini Kit Qiagen cat#74134 plus additional on column Dnase treatment
Size selection method
dual SPRI
Strand specificity
Strand-specific (forward)
Controls

Attribution

Genomics of Gene Regulation Project
Lab
Tim Reddy, Duke
Award
U01HG007900 (Tim Reddy, Duke)
Project
GGR
External resources
Aliases
tim-reddy:t10_rna_seq
Date submitted
March 3, 2016
Date released
May 5, 2016
Treatment Time Series
ENCSR897XFT 

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
11Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 10 hoursENCBS405EQXENCLB451LSM
31Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 10 hoursENCBS146CRNENCLB101JNT
41Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 10 hoursENCBS462FXSENCLB809HNX
21Homo sapiens A549 cell line treated with 100 nM dexamethasone (agonist) for 10 hoursENCBS721HGMENCLB565JKN