Experiment summary for ENCSR219DFA

doi:10.17989/ENCSR219DFA

Summary

Status
released
Assay
RNA-seq (total RNA-seq)
Biosample summary
Mus musculus strain C57BL/6J macrophage nuclear fraction
Biosample Type
primary cell
Replication type
isogenic
Description
Ribo depletion RNA-seq on nucleoplasmic fraction RNA which is part of BMDM cell, stimulated with 100 ng/ml Lipid A for 10 minute.
Nucleic acid type
RNA
Depleted in
rRNA
Library starting quantity
500ng [2-1]1µg [1-1]
Size range
250-400
Fragmentation methods
see document
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)

Attribution

Genomics of Gene Regulation Project
Lab
Alexander Hoffmann, UCLA
Award
U01HG007912 (Alexander Hoffmann, UCLA)
Project
GGR
External resources
Aliases
alexander-hoffmann:Nuc.Naive.010.exp
Date submitted
January 20, 2017
Date released
November 20, 2018

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
21Mus musculus strain C57BL/6J macrophage primary cell nuclear fractionENCBS105BSRENCLB693HZY
11Mus musculus strain C57BL/6J macrophage primary cell nuclear fractionENCBS565OIKENCLB898FOH