Experiment summary for ENCSR216NEG
doi:10.17989/ENCSR216NEG
Summary
- Status
- released
- Assay
- polyA plus RNA-seq
- Biosample summary
- Mus musculus strain B6NCrl limb tissue embryo (14.5 days)
- Biosample Type
- tissue
- Replication type
- isogenic
- Description
- PolyA RNA-Seq from oligo-dT primed Total RNA on embryonic 14.5 day mouse limb
- Nucleic acid type
- polyadenylated mRNA
- RNA integrity number
- 9.4 [2-1]9.9 [1-1]
- Library starting quantity
- 10ng
- Size range
- >200
- Extraction method
- Ambion mirVana
- Fragmentation methods
- chemical (Nextera tagmentation)
- Size selection method
- SPRI beads
- Strand specificity
- unstranded
- Spike-ins datasets
- ENCSR884LPM
- Platform
- Illumina HiSeq 2500
Attribution
ENCODE3 project
- Lab
- Barbara Wold, Caltech
- Award
- U54HG006998 (Richard Myers, HAIB)
- Project
- ENCODE
- External resources
- References
- Date submitted
- December 11, 2014
- Date released
- December 17, 2014
- Reference Epigenome
- ENCSR705YBY
- Organism Development Series
- ENCSR098WGB , ENCSR407MLM , ENCSR902MLV
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
---|---|---|---|---|
2 | 1 | Mus musculus strain B6NCrl mixed sex embryo (14.5 days) limb tissue | ENCBS471PIU | ENCLB949YQN |
1 | 1 | Mus musculus strain B6NCrl mixed sex embryo (14.5 days) limb tissue | ENCBS300NIO | ENCLB906TKN |