Experiment summary for ENCSR215QTA
doi:10.17989/ENCSR215QTA
Summary
- Status
- released
- Assay
- whole-genome shotgun bisulfite sequencing (WGBS)
- Biosample summary
- Mus musculus strain B6NCrl midbrain tissue embryo (15.5 days)
- Biosample Type
- tissue
- Replication type
- isogenic
- Description
- Whole genome bisulfite-seq on embryonic 15.5 day mouse midbrain
- Nucleic acid type
- DNA
- Size range
- 180-500
- Lysis method
- QIAGEN DNeasy Blood & Tissue Kit
- Fragmentation methods
- shearing (Covaris S2)
- Size selection method
- Sera-mag SpeedBeads
- Platform
- Illumina HiSeq 2500
Attribution
ENCODE3 project
- Lab
- Joe Ecker, Salk
- Award
- U54HG006997 (Bing Ren, UCSD)
- Project
- ENCODE
- External resources
- Aliases
- joe-ecker:E15_5_MB
- Date submitted
- April 29, 2015
- Date released
- June 23, 2015
- Organism Development Series
- ENCSR414HVF
- Reference Epigenome
- ENCSR453LDG
- Submitter comment
- Fastq files ENCFF208JMN, ENCFF495GRC, and ENCFF618YSU contain reads of mixed lengths. Read lengths followed by respective number of reads: ENCFF208JMN (108, 211151094), (130, 425081487); ENCFF495GRC (109, 211199336), (130, 215552816), (139, 422210237); ENCFF618YSU (130, 199128027), (139, 214570449).
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
---|---|---|---|---|
2 | 1 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) midbrain tissue | ENCBS381PEJ | ENCLB624VSR |
1 | 1 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) midbrain tissue | ENCBS190ETJ | ENCLB224DGR |
1 | 2 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) midbrain tissue | ENCBS190ETJ | ENCLB813KHU |
2 | 2 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) midbrain tissue | ENCBS381PEJ | ENCLB064PKH |