Experiment summary for ENCSR209ZYI

Summary

Status
released
Assay
ChIP-seq
Biosample summary
Caenorhabditis elegans strain OP344 (unc-119(tm4063); wgIs344 [F49E12.6::TY1::EGFP::3xFLAG + unc-119(+)]) whole organism L1 larva (6 hours) at L1 larva starved after bleaching stage genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic worms expressing EFL-3-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation method
sonication (Bioruptor generic)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
valerie-reinke:EFL-3_OP344_L1
Date submitted
September 17, 2018
Date released
September 19, 2018

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
21strain OP344 (unc-119(tm4063); wgIs344 [F49E12.6::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex L1 larva (6 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting eGFP-efl-3ENCBS980YFXENCGM759HZZENCAB728YTOENCLB982KHX
11strain OP344 (unc-119(tm4063); wgIs344 [F49E12.6::TY1::EGFP::3xFLAG + unc-119(+)]) mixed sex L1 larva (6 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting eGFP-efl-3ENCBS770AMNENCGM759HZZENCAB728YTOENCLB358ZGN