Experiment summary for ENCSR204WOO
doi:10.17989/ENCSR204WOO
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- sox-4
- Biosample summary
- Caenorhabditis elegans strain OP517 (unc-119(tm4063) III; wgIs517) whole organism late embryo 4 hours post synchronization using egg bleaching genetically modified (insertion) using bombardment targeting C. elegans sox-4
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing SOX-4-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation methods
- sonication (generic microtip)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000, Illumina HiSeq 2000
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:SOX-4-OP517-LE
- Date submitted
- October 6, 2017
- Date released
- November 6, 2017
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Caenorhabditis elegans strain OP517 (unc-119(tm4063) III; wgIs517) mixed sex late embryo 4 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans sox-4 | ENCBS133PPJ | ENCGM544OTR | ENCAB728YTO | ENCLB799QST |
| 2 | 1 | Caenorhabditis elegans strain OP517 (unc-119(tm4063) III; wgIs517) mixed sex late embryo 4 hours post synchronization using egg bleaching whole organism genetically modified (insertion) using bombardment targeting C. elegans sox-4 | ENCBS508TJU | ENCGM544OTR | ENCAB728YTO | ENCLB615AUU |