Experiment summary for ENCSR193PSI
doi:10.17989/ENCSR193PSI
Alternate accession: ENCSR763RKN
Summary
- Status
- released
- Assay
- shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
- Target
- bsh
- Biosample summary
- Drosophila melanogaster strain bsh_shRNA x da-Gal4 whole organism embryo 16-18 hours post synchronization at egg laying stage expressing RNAi targeting D. melanogaster bsh
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Nucleic acid type
- polyadenylated mRNA
- Size range
- 350-600
- Extraction method
- Trizol
- Fragmentation methods
- shearing (Covaris generic)
- Strand specificity
- unstranded
- Platform
- Illumina NextSeq 500
- Controls
Attribution
modERN project
- Lab
- Susan Celniker, LBNL
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- Aliases
- susan-celniker:bsh_16-18
- Date submitted
- March 14, 2019
- Date released
- April 25, 2019
- Submitter comment
- Processed data is currently not available for these experiments, but will be added in the future.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 1 | 1 | Drosophila melanogaster strain bsh_shRNA x da-Gal4 embryo 16-18 hours post synchronization at egg laying stage whole organism expressing RNAi targeting D. melanogaster bsh | ENCBS668HPM | ENCGM027YSK | ENCLB801FCY |
| 2 | 1 | Drosophila melanogaster strain bsh_shRNA x da-Gal4 embryo 16-18 hours post synchronization at egg laying stage whole organism expressing RNAi targeting D. melanogaster bsh | ENCBS336PXM | ENCGM027YSK | ENCLB451VOC |