Experiment summary for ENCSR184CWK

doi:10.17989/ENCSR184CWK

Summary

Status
archived
Assay
single-cell RNA sequencing assay (scRNA-seq)
Biosample summary
Homo sapiens GM12878
Biosample Type
cell line
Replication type
unreplicated
Description
RNA-seq of a single GM12878 cell
Nucleic acid type
RNA
Library starting quantity
1cells
Size range
>200
Lysis method
72 degrees for 3 minutes in the presence of Triton
Fragmentation methods
chemical (Nextera tagmentation)
Size selection method
SPRI beads
Strand specificity
unstranded
Spike-ins datasets
ENCSR013YHQ
Controls

Attribution

ENCODE3 project
Lab
Barbara Wold, Caltech
Award
U54HG006998 (Richard Myers, HAIB)
Project
ENCODE
External resources
Aliases
barbara-wold:single-cell-13289-exp
Date submitted
May 13, 2014
Date released
June 30, 2014
Submitter comment
Estimates of technical measurement error from low input mass RNA-seq libraries.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
11Homo sapiens GM12878 cell lineENCBS381VEVENCLB206JYT