Experiment summary for ENCSR184CKS

doi:10.17989/ENCSR184CKS

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
Fer1
Biosample summary
Drosophila melanogaster strain Fer1-GFP whole organism embryo (6-24 hours) genetically modified (insertion) using site-specific recombination targeting D. melanogaster Fer1
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing Fer1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:Fer1-GFP
Date submitted
December 21, 2016
Date released
January 10, 2017
Submitter comment
ENCFF170JQM, ENCFF875DRD were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21Drosophila melanogaster strain Fer1-GFP mixed sex embryo (6-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Fer1ENCBS586AHUENCGM155TLAENCAB728YTOENCLB435WYI
11Drosophila melanogaster strain Fer1-GFP mixed sex embryo (6-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster Fer1ENCBS236CKMENCGM155TLAENCAB728YTOENCLB264BFG