Experiment summary for ENCSR181KQJ
doi:10.17989/ENCSR181KQJ
Alternate accession: ENCSR488BHJ
Summary
- Status
- released
- Assay
- RNA-seq (total RNA-seq)
- Biosample summary
- Mus musculus strain C57BL/6J G1E-ER4 treated with 10 nM 17β-estradiol for 24 hours
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- PSU mouse G1E-ER4+E2 RNA RNA-seq
- Treatments
- 10 nM 17β-estradiol (CHEBI:16469) for 24 hours
- Nucleic acid type
- RNA
- Library starting quantity
- 1ng
- Size range
- >200
- Fragmentation methods
- chemical (Nextera tagmentation)
- Size selection method
- SPRI beads
- Strand specificity
- Strand-specific (reverse)
- Spike-ins datasets
- ENCSR013YHQ, ENCSR156CIL
- Platform
- Illumina HiSeq 2000
Attribution
ENCODE3 project
- Lab
- Ross Hardison, PennState
- Award
- U54HG006998 (Richard Myers, HAIB)
- Project
- ENCODE
- External resources
- Date submitted
- October 29, 2014
- Date released
- November 20, 2014
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
---|---|---|---|---|
1 | 1 | Mus musculus strain C57BL/6J G1E-ER4 cell line treated with 10 nM 17β-estradiol for 24 hours | ENCBS158MXR | ENCLB806ISB |
2 | 1 | Mus musculus strain C57BL/6J G1E-ER4 cell line treated with 10 nM 17β-estradiol for 24 hours | ENCBS214CAT | ENCLB003LKL |