Experiment summary for ENCSR176PDW

Summary

Status
released
Assay
ChIP-seq
Target
eGFP-Six4
Biosample summary
Drosophila melanogaster attP-40 whole organism embryo (0-16 hours) genetically modified using site-specific recombination
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing Six4-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation method
sonication (Bioruptor generic)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:Six4-GFP
Date submitted
August 3, 2016
Date released
September 29, 2016
Submitter comment
ENCFF451BWY, ENCFF370LHY were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files.

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
11strain Six4-GFP mixed sex embryo (0-16 hours) whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-Six4ENCBS941PGQENCGM081BFEENCAB728YTOENCLB372TME
31strain Six4-GFP mixed sex embryo (0-16 hours) whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-Six4ENCBS398WTTENCGM081BFEENCAB728YTOENCLB438SEX