Experiment summary for ENCSR172KOI

Summary

Status
released
Assay
ChIP-seq
Target
eGFP-kay
Biosample summary
Drosophila melanogaster VK00037 whole organism embryo (0-24 hours) genetically modified using site-specific recombination
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing kay-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation method
sonication (Bioruptor generic)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:kay-GFP-EM
Date submitted
April 10, 2016
Date released
July 6, 2016
Submitter comment
ENCFF068BPZ, ENCFF828APS, ENCFF760DFJ, and ENCFF839RZM were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files.

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
21strain kay-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-kayENCBS025NWAENCGM972TWGENCAB728YTOENCLB652JGR
41strain kay-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-kayENCBS255JFWENCGM972TWGENCAB728YTOENCLB363HSV