Experiment summary for ENCSR167BID

doi:10.17989/ENCSR167BID

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
bcd
Biosample summary
Drosophila melanogaster strain 1T-GFP whole organism embryo (0-24 hours) genetically modified (insertion) using site-specific recombination targeting D. melanogaster bcd
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing bcd-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:bcd-GFP
Date submitted
December 21, 2016
Date released
January 11, 2017
Submitter comment
ENCFF291PJD, ENCFF757RAY, ENCFF382YPN, ENCFF201OPZ were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11Drosophila melanogaster strain 1T-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster bcdENCBS952BGSENCGM618KNNENCAB728YTOENCLB015IHK
31Drosophila melanogaster strain 1T-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster bcdENCBS952CGKENCGM618KNNENCAB728YTOENCLB619BOI