Experiment summary for ENCSR158PGO

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
lsy-27
Biosample summary
Caenorhabditis elegans strain OP401 whole organism hermaphrodite young adult genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing LSY-27-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
E-Gel
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:LSY-27-OP401-yAd
Date submitted
July 30, 2015
Date released
November 22, 2017
Submitter comment
This experiment was completed during modENCODE and has lower read depth than the current modERN standard.

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21strain OP401 hermaphrodite young adult whole organism genetically modified (insertion) using bombardment targeting lsy-27ENCBS937BINENCGM413WQCENCAB435FBQENCLB278CMP
31strain OP401 hermaphrodite young adult whole organism genetically modified (insertion) using bombardment targeting lsy-27ENCBS801YMYENCGM413WQCENCAB435FBQENCLB581LON