Experiment summary for ENCSR154LSU
doi:10.17989/ENCSR154LSU
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- akir-1
- Biosample summary
- Caenorhabditis elegans strain IG1654 (frSi12[pNP157(akir-1p::AKIR-1::GFP)]II) whole organism young adult (70 hours) at L1 larva starved after bleaching stage genetically modified (insertion) using CRISPR targeting C. elegans akir-1
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic worms expressing akir-1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:AKIR-1_IG1654_yAd
- Date submitted
- June 11, 2018
- Date released
- June 13, 2018
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 3 | 1 | Caenorhabditis elegans strain IG1654 (frSi12[pNP157(akir-1p::AKIR-1::GFP)]II) mixed sex young adult (70 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using CRISPR targeting C. elegans akir-1 | ENCBS847YWI | ENCGM524HUG | ENCAB728YTO | ENCLB100HBA |
| 4 | 1 | Caenorhabditis elegans strain IG1654 (frSi12[pNP157(akir-1p::AKIR-1::GFP)]II) mixed sex young adult (70 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using CRISPR targeting C. elegans akir-1 | ENCBS066PNQ | ENCGM524HUG | ENCAB728YTO | ENCLB733POB |