Experiment summary for ENCSR154LSU

Summary

Status
released
Assay
ChIP-seq
Biosample summary
Caenorhabditis elegans strain IG1654 (frSi12[pNP157(akir-1p::AKIR-1::GFP)]II) whole organism young adult (70 hours) at L1 larva starved after bleaching stage genetically modified using CRISPR
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic worms expressing akir-1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation method
sonication (Bioruptor generic)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:AKIR-1_IG1654_yAd
Date submitted
June 11, 2018
Date released
June 13, 2018

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
31strain IG1654 (frSi12[pNP157(akir-1p::AKIR-1::GFP)]II) mixed sex young adult (70 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using CRISPR targeting eGFP-akir-1ENCBS847YWIENCGM524HUGENCAB728YTOENCLB100HBA
41strain IG1654 (frSi12[pNP157(akir-1p::AKIR-1::GFP)]II) mixed sex young adult (70 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using CRISPR targeting eGFP-akir-1ENCBS066PNQENCGM524HUGENCAB728YTOENCLB733POB