Experiment summary for ENCSR149GUT
doi:10.17989/ENCSR149GUT
Summary
- Status
- released
- Assay
- whole-genome shotgun bisulfite sequencing (WGBS)
- Biosample summary
- Mus musculus strain B6NCrl heart tissue embryo (13.5 days)
- Biosample Type
- tissue
- Replication type
- isogenic
- Description
- Whole genome bisulfite-seq on embryonic 13.5 day mouse heart
- Nucleic acid type
- DNA
- Size range
- 180-500
- Lysis method
- QIAGEN DNeasy Blood & Tissue Kit
- Fragmentation methods
- shearing (Covaris S2)
- Size selection method
- Sera-mag SpeedBeads
- Platform
- Illumina HiSeq 2500
Attribution
ENCODE3 project
- Lab
- Joe Ecker, Salk
- Award
- U54HG006997 (Bing Ren, UCSD)
- Project
- ENCODE
- External resources
- Aliases
- joe-ecker:E13_5_HT
- Date submitted
- August 14, 2015
- Date released
- August 18, 2015
- Organism Development Series
- ENCSR781CPK
- Reference Epigenome
- ENCSR107EDN
- Submitter comment
- Fastq files ENCFF070DXX, ENCFF652QDS, ENCFF136ISB, and ENCFF287MQU contain reads of mixed lengths. Read lengths followed by respective number of reads: ENCFF070DXX (130, 201833048), (136, 424817193); ENCFF652QDS (130, 208410381), (136, 223741495); ENCFF136ISB (130, 208887880), (136, 428245778); ENCFF287MQU (130, 465743068), (136, 207369124).
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
---|---|---|---|---|
2 | 1 | Mus musculus strain B6NCrl mixed sex embryo (13.5 days) heart tissue | ENCBS814PLT | ENCLB054AXP |
1 | 1 | Mus musculus strain B6NCrl mixed sex embryo (13.5 days) heart tissue | ENCBS490ZWZ | ENCLB909QYX |
2 | 2 | Mus musculus strain B6NCrl mixed sex embryo (13.5 days) heart tissue | ENCBS814PLT | ENCLB086MBV |
1 | 2 | Mus musculus strain B6NCrl mixed sex embryo (13.5 days) heart tissue | ENCBS490ZWZ | ENCLB576AVF |