Experiment summary for ENCSR103NNB

Summary

Status
released
Assay
CRISPR genome editing followed by RNA-seq (CRISPR RNA-seq)
Target
SDAD1
Biosample summary
Homo sapiens K562 genetically modified using CRISPR
Biosample Type
cell line
Replication type
isogenic
Description
RNA-seq on K562 cells treated with a CRISPR gRNA against SDAD1. (SDAD1-BGKcLV06)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
10
Size range
>200
Lysis method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation method
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific
Spike-ins datasets
ENCSR449DXG
Controls

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
Aliases
brenton-graveley:SDAD1-BGKcLV06
Date submitted
November 20, 2017
Date released
December 9, 2017
Tags
Search for all Experiment with internal tag ENCORE

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsLibrary
11K562 cell line genetically modified (deletion) using CRISPR targeting SDAD1ENCBS880QCJENCGM672AIUENCLB154DOT
21K562 cell line genetically modified (deletion) using CRISPR targeting SDAD1ENCBS367HEPENCGM672AIUENCLB125DBW