Experiment summary for ENCSR085TSA
doi:10.17989/ENCSR085TSA
Summary
- Status
- released
- Assay
- RNA-seq (total RNA-seq)
- Biosample summary
- Mus musculus strain C57BL/6J dendritic cell originated from whole organism treated with 100 ng/mL lipopolysaccharide for 4 hours
- Biosample Type
- in vitro differentiated cells
- Replication type
- isogenic
- Description
- RNA-Seq
- Treatments
- 100 ng/mL lipopolysaccharide (CHEBI:16412) for 4 hours
- Nucleic acid type
- RNA
- Size range
- 175-500
- Extraction method
- RNeasy Plus Mini Kit Qiagen cat#74134 plus additional on column Dnase treatment
- Strand specificity
- Strand-specific (forward)
- Platform
- Illumina NextSeq 500
Attribution
Genomics of Gene Regulation Project
- Lab
- Manuel Garber, UMass
- Award
- U01HG007910 (Jeremy Luban, UMass)
- Project
- GGR
- External resources
- Aliases
- manuel-garber:mDC-4h_LPS_RNA-seq
- Date submitted
- January 18, 2017
- Date released
- January 19, 2017
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 1 | 1 | Mus musculus strain C57BL/6J dendritic in vitro differentiated cells originated from whole organism treated with 100 ng/mL lipopolysaccharide for 4 hours | ENCBS216NIS | ENCLB219HCZ |
| 2 | 1 | Mus musculus strain C57BL/6J dendritic in vitro differentiated cells originated from whole organism treated with 100 ng/mL lipopolysaccharide for 4 hours | ENCBS230UHY | ENCLB542YDS |