Experiment summary for ENCSR081BBL

doi:10.17989/ENCSR081BBL

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
daf-16
Biosample summary
Caenorhabditis elegans strain ZM7247 (daf-16(mu86);daf-2(e1370) III; hpSi4II (Pmyo-3 GFP::daf-16a)) whole organism hermaphrodite L4 larva 54 hours post synchronization at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans daf-16
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing DAF-16-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:DAF-16-ZM7247-L4
Date submitted
July 29, 2015
Date released
May 10, 2016
Submitter comment
ENCFF516WOU, ENCFF291DCA, ENCFF360ZYQ, ENCFF174GOF were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21Caenorhabditis elegans strain ZM7247 (daf-16(mu86);daf-2(e1370) III; hpSi4II (Pmyo-3 GFP::daf-16a)) hermaphrodite L4 larva 54 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans daf-16ENCBS773BAMENCGM225SBVENCAB728YTOENCLB273NWN
11Caenorhabditis elegans strain ZM7247 (daf-16(mu86);daf-2(e1370) III; hpSi4II (Pmyo-3 GFP::daf-16a)) hermaphrodite L4 larva 54 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans daf-16ENCBS616VAUENCGM225SBVENCAB728YTOENCLB205BGU