Experiment summary for ENCSR081BBL
doi:10.17989/ENCSR081BBL
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- daf-16
- Biosample summary
- Caenorhabditis elegans strain ZM7247 (daf-16(mu86);daf-2(e1370) III; hpSi4II (Pmyo-3 GFP::daf-16a)) whole organism hermaphrodite L4 larva 54 hours post synchronization at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans daf-16
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq of transgenic worms expressing DAF-16-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Fragmentation methods
- sonication (generic microtip)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 2500
- Controls
Attribution
modERN project
- Lab
- Valerie Reinke, Yale
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:DAF-16-ZM7247-L4
- Date submitted
- July 29, 2015
- Date released
- May 10, 2016
- Submitter comment
- ENCFF516WOU, ENCFF291DCA, ENCFF360ZYQ, ENCFF174GOF were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 2 | 1 | Caenorhabditis elegans strain ZM7247 (daf-16(mu86);daf-2(e1370) III; hpSi4II (Pmyo-3 GFP::daf-16a)) hermaphrodite L4 larva 54 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans daf-16 | ENCBS773BAM | ENCGM225SBV | ENCAB728YTO | ENCLB273NWN |
| 1 | 1 | Caenorhabditis elegans strain ZM7247 (daf-16(mu86);daf-2(e1370) III; hpSi4II (Pmyo-3 GFP::daf-16a)) hermaphrodite L4 larva 54 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans daf-16 | ENCBS616VAU | ENCGM225SBV | ENCAB728YTO | ENCLB205BGU |