Experiment summary for ENCSR075AVX

doi:10.17989/ENCSR075AVX

Summary

Status
released
Assay
ChIP-seq (Histone ChIP-seq)
Target
H3K4me3
Biosample summary
Homo sapiens dendritic cell treated with 100 ng/mL lipopolysaccharide for 30 minutes
Biosample Type
in vitro differentiated cells
Replication type
unreplicated
Description
ChIP-seq
Treatments
100 ng/mL lipopolysaccharide (CHEBI:16412) for 30 minutes
Nucleic acid type
DNA
Size range
150-400
Extraction method
TruChIP chromatin shearing reagent kit Covaris cat# 520154, sonication Covaris M220, IP (home protocol), DNA isolation with QIAquick PCR Purification Kit cat# 28104.

Attribution

Genomics of Gene Regulation Project
Lab
Manuel Garber, UMass
Award
U01HG007910 (Jeremy Luban, UMass)
Project
GGR
External resources
Aliases
manuel-garber:F33_MDDC_H3K4me3_Lps_30m_ChIP-seq
Date submitted
January 18, 2017
Date released
January 19, 2017

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Antibody
Library
11Homo sapiens dendritic in vitro differentiated cells treated with 100 ng/mL lipopolysaccharide for 30 minutesENCBS550GVGENCAB884CKIENCLB751CXO