Experiment summary for ENCSR075AVX
Summary
- Status
- released
- Assay
- ChIP-seq
- Target
- H3K4me3
- Biosample summary
- Homo sapiens dendritic cell treated with 100 ng/mL lipopolysaccharide for 30 minutes
- Biosample Type
- in vitro differentiated cells
- Replication type
- unreplicated
- Description
- ChIP-seq
- Treatments
- 100 ng/mL lipopolysaccharide (CHEBI:16412) for 30 minute
- Nucleic acid type
- DNA
- Size range
- 150-400
- Extraction method
- TruChIP chromatin shearing reagent kit Covaris cat# 520154, sonication Covaris M220, IP (home protocol), DNA isolation with QIAquick PCR Purification Kit cat# 28104.
- Strand specificity
- Non-strand-specific
- Platform
- Illumina NextSeq 500
Attribution
Genomics of Gene Regulation Project
- Lab
- Manuel Garber, UMass
- Award
- U01HG007910 (Jeremy Luban, UMass)
- Project
- GGR
- External resources
- Aliases
- manuel-garber:F33_MDDC_H3K4me3_Lps_30m_ChIP-seq
- Date submitted
- January 18, 2017
- Date released
- January 19, 2017
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Antibody | Library |
---|---|---|---|---|---|
1 | 1 | dendritic in vitro differentiated cells treated with 100 ng/mL lipopolysaccharide for 30 minutes | ENCBS550GVG | ENCAB884CKI | ENCLB751CXO |