Experiment summary for ENCSR070MVF

Summary

Status
released
Assay
single cell isolation followed by RNA-seq (scRNA-seq)
Biosample summary
Mus musculus C57BL/6 forelimb embryo (15.5 days)
Biosample Type
single cell
Replication type
unreplicated
Description
C1 single cell e15.5 mouse forelimb.
Nucleic acid type
RNA
Library starting quantity
1cells
Size range
300-500
Lysis method
Clontech UltraLow for Illumina sequencing
Extraction method
C1 fluidigm
Fragmentation methods
chemical (Nextera tagmentation)
Size selection method
SPRI beads
Spike-ins datasets
ENCSR535LMC
Library construction platform
Fluidigm C1 System

Attribution

ENCODE4 project
Lab
Barbara Wold, Caltech
Award
UM1HG009443 (Barbara Wold, Caltech)
Project
ENCODE
Aliases
barbara-wold:c1_e15.5_mouse_limb_run14_20048_E8
Date released
February 12, 2020

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
11strain B6NCrl embryo (15.5 days) forelimb single cellENCBS207HJLENCLB055WNY