Experiment summary for ENCSR059TJM

doi:10.17989/ENCSR059TJM

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
CG3995
Biosample summary
Drosophila melanogaster strain CG3995-GFP whole organism embryo (0-24 hours) genetically modified (insertion) using site-specific recombination targeting D. melanogaster CG3995
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing CG3995-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation methods
sonication (Bioruptor generic)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:CG3995-GFP
Date submitted
August 3, 2016
Date released
September 29, 2016
Submitter comment
ENCFF553XRI, ENCFF980GCK were revoked because a software bug accidentally truncated the file content. The submitters have provided replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11Drosophila melanogaster strain CG3995-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster CG3995ENCBS050GOIENCGM167CTTENCAB728YTOENCLB781COA
31Drosophila melanogaster strain CG3995-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster CG3995ENCBS878BKYENCGM167CTTENCAB728YTOENCLB602MRY
21Drosophila melanogaster strain CG3995-GFP mixed sex embryo (0-24 hours) whole organism genetically modified (insertion) using site-specific recombination targeting D. melanogaster CG3995ENCBS529WGAENCGM167CTTENCAB728YTOENCLB457NHA