Experiment summary for ENCSR052SDT

doi:10.17989/ENCSR052SDT

Summary

Status
released
Assay
RNA-seq (total RNA-seq)
Biosample summary
Homo sapiens activated CD4-positive, alpha-beta T cell male adult (35 years) treated with anti-CD3 and anti-CD28 coated beads
Biosample Type
primary cell
Replication type
isogenic
Description
CD4 N (RA+ MACS sort)Th0 (no diff Cytokines)(day 13)+beads (1:3) day 1
Treatments
anti-CD3 and anti-CD28 coated beads
Nucleic acid type
RNA
RNA integrity number
9
Library starting quantity
300ng
Average fragment size
274 [1-1]281 [2-1]
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR597UQL

Attribution

ENCODE4 project
Lab
Barbara Wold, Caltech
Award
UM1HG009443 (Barbara Wold, Caltech)
Project
ENCODE
Date released
July 14, 2022

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Library
11Homo sapiens male adult (35 years) activated CD4-positive, alpha-beta T primary cell treated with anti-CD3 and anti-CD28 coated beadsENCBS238ZBDENCLB646RRY
21Homo sapiens male adult (35 years) activated CD4-positive, alpha-beta T primary cell treated with anti-CD3 and anti-CD28 coated beadsENCBS242UJRENCLB454DSL