Experiment summary for ENCSR051GNG
doi:10.17989/ENCSR051GNG
Alternate accession: ENCSR438NYW
Summary
- Status
- released
- Assay
- shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
- Target
- Blimp-1
- Biosample summary
- Drosophila melanogaster strain Blimp-1_shRNA x da-Gal4 whole organism embryo 0-1.5 hours post synchronization at egg laying stage expressing RNAi targeting D. melanogaster Blimp-1
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Nucleic acid type
- RNA
- Depleted in
- polyadenylated mRNA
- Size range
- 350-600
- Extraction method
- Trizol
- Fragmentation methods
- shearing (Covaris generic)
- Strand specificity
- unstranded
- Platform
- Illumina NextSeq 500
- Controls
Attribution
modERN project
- Lab
- Susan Celniker, LBNL
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- Aliases
- susan-celniker:Blimp-1_0-1.5
- Date submitted
- March 14, 2019
- Date released
- April 25, 2019
- Submitter comment
- Processed data is currently not available for these experiments, but will be added in the future.
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Library |
|---|---|---|---|---|---|
| 2 | 1 | Drosophila melanogaster strain Blimp-1_shRNA x da-Gal4 embryo 0-1.5 hours post synchronization at egg laying stage whole organism expressing RNAi targeting D. melanogaster Blimp-1 | ENCBS887YVP | ENCGM673GIL | ENCLB904FHW |
| 1 | 1 | Drosophila melanogaster strain Blimp-1_shRNA x da-Gal4 embryo 0-1.5 hours post synchronization at egg laying stage whole organism expressing RNAi targeting D. melanogaster Blimp-1 | ENCBS006PSU | ENCGM673GIL | ENCLB709SNO |