Experiment summary for ENCSR049FHU

Summary

Status
released
Assay
ChIP-seq
Biosample summary
Caenorhabditis elegans strain OP749 (wgIs749 [tlf-1::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) whole organism young adult (65.5 hours) at L1 larva starved after bleaching stage genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic worms expressing tlf-1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation method
sonication (Bioruptor generic)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:TLF-1_OP749_yAd
Date submitted
June 11, 2018
Date released
June 12, 2018

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
11strain OP749 (wgIs749 [tlf-1::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) mixed sex young adult (65.5 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting eGFP-tlf-1ENCBS967LSPENCGM853GKKENCAB728YTOENCLB647KSA
21strain OP749 (wgIs749 [tlf-1::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) mixed sex young adult (65.5 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting eGFP-tlf-1ENCBS367YVLENCGM853GKKENCAB728YTOENCLB952CVU