Experiment summary for ENCSR049FHU
doi:10.17989/ENCSR049FHU
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- tlf-1
- Biosample summary
- Caenorhabditis elegans strain OP749 (wgIs749 [tlf-1::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) whole organism young adult (65.5 hours) at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans tlf-1
- Biosample Type
- whole organisms
- Replication type
- isogenic
- Description
- ChIP-seq on transgenic worms expressing tlf-1-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
- Nucleic acid type
- DNA
- Size range
- 200-800
- Fragmentation methods
- sonication (Bioruptor generic)
- Size selection method
- SPRI beads
- Platform
- Illumina HiSeq 4000
- Controls
Attribution
modERN project
- Lab
- Kevin White, UChicago
- Award
- U41HG007355 (Robert Waterston, UW)
- Project
- modERN
- External resources
- Aliases
- kevin-white:TLF-1_OP749_yAd
- Date submitted
- June 11, 2018
- Date released
- June 12, 2018
- Factorbook
Encyclopedia Integration
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 1 | 1 | Caenorhabditis elegans strain OP749 (wgIs749 [tlf-1::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) mixed sex young adult (65.5 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans tlf-1 | ENCBS967LSP | ENCGM853GKK | ENCAB728YTO | ENCLB647KSA |
| 2 | 1 | Caenorhabditis elegans strain OP749 (wgIs749 [tlf-1::TY1::EGFP::3xFLAG + unc-119(+)];unc-119 (tm4063)) mixed sex young adult (65.5 hours) at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans tlf-1 | ENCBS367YVL | ENCGM853GKK | ENCAB728YTO | ENCLB952CVU |