Experiment summary for ENCSR041UPG
doi:10.17989/ENCSR041UPG
Summary
- Status
- released
- Assay
- ChIA-PET
- Target
- POLR2A
- Biosample summary
- Homo sapiens activated CD8-positive, alpha-beta memory T cell male adult (30 years) treated with anti-CD3 and anti-CD28 coated beads for 36 hours
- Biosample Type
- primary cell
- Replication type
- unreplicated
- Description
- RNAPII ChIA-PET in Memory_CD8_Activated_Day2
- Treatments
- anti-CD3 and anti-CD28 coated beads for 36 hours
- Nucleic acid type
- DNA
- Size range
- 350-600
- Lysis method
- SDS
- Fragmentation methods
- sonication (Branson Sonifier 250)
- Size selection method
- BluePippin
- Platform
- Illumina MiSeq, Illumina NovaSeq 6000
Attribution
ENCODE4 project
- Lab
- Yijun Ruan, JAX
- Award
- UM1HG009409 (Yijun Ruan, JAX)
- Project
- ENCODE
- External resources
- Aliases
- yijun-ruan:Memory_CD8_Activated_Day2_RNAPII
- Date submitted
- August 2, 2020
- Date released
- October 6, 2021
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Antibody | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens male adult (30 years) activated CD8-positive, alpha-beta memory T primary cell treated with anti-CD3 and anti-CD28 coated beads for 36 hours | ENCBS629LOT | ENCAB725WBH | ENCLB629AXC |