Experiment summary for ENCSR031RRO

doi:10.17989/ENCSR031RRO

Summary

Status
released
Assay
shRNA knockdown followed by RNA-seq (shRNA RNA-seq)
Control type
non-specific target
Biosample summary
Homo sapiens K562 expressing RNAi
Biosample Type
cell line
Replication type
isogenic
Description
Control shRNA against no target in K562 cells followed by RNA-seq. (NT_BGKLV25B)
Nucleic acid type
polyadenylated mRNA
RNA integrity number
10 [1-1]9.9 [2-1]
Size range
>200
Extraction method
Maxwell 16 LEV simpleRNA Cells Kit (Promega cat#: AS1270)
Fragmentation methods
chemical (Illumina TruSeq)
Size selection method
SPRI beads
Strand specificity
Strand-specific (reverse)
Spike-ins datasets
ENCSR358OMW

Attribution

ENCODE3 project
Lab
Brenton Graveley, UConn
Award
U54HG007005 (Brenton Graveley, UConn)
Project
ENCODE
External resources
Aliases
brenton-graveley:NT_BGKLV25B
Date submitted
May 26, 2016
Date released
June 13, 2016
Gene Silencing Series
ENCSR730QGA 
, ENCSR369EIU 
, ENCSR253WFB 
Tags
Visit collection page for ENCORESearch for all Experiment with internal tag ENCYCLOPEDIAv2

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Library
11Homo sapiens K562 cell line expressing RNAiENCBS745CIFENCGM266AWPENCLB778LQL
21Homo sapiens K562 cell line expressing RNAiENCBS140NYGENCGM266AWPENCLB796OAN