Experiment summary for ENCSR028XNW

Summary

Status
released
Assay
ChIP-seq
Target
Control
Biosample summary
Caenorhabditis elegans strain OP700 (wgIs700(drap-1::TY1 EGFP 3xFLAG(p_wTRG5_insertion_c_0086_1E01);unc-119(+));unc-119 (tm4063)) whole organism mixed stage (embryo) (3 hours) using egg bleaching genetically modified using bombardment
Biosample Type
whole organisms
Replication type
isogenic
Description
This is the ChIP-seq control of transgenic worms expressing drap-1-eGFP fusion proteins.
Nucleic acid type
DNA
Size range
200-800
Fragmentation method
sonication (Bioruptor generic)
Size selection method
SPRI
Strand specificity
Non-strand-specific

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:DRAP-1_OP700_EM-INPUT
Date submitted
May 30, 2018
Date released
June 12, 2018

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsLibrary
21strain OP700 (wgIs700(drap-1::TY1 EGFP 3xFLAG(p_wTRG5_insertion_c_0086_1E01);unc-119(+));unc-119 (tm4063)) mixed sex mixed stage (embryo) (3 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting eGFP-drap-1ENCBS947USYENCGM126PQFENCLB343FYP
11strain OP700 (wgIs700(drap-1::TY1 EGFP 3xFLAG(p_wTRG5_insertion_c_0086_1E01);unc-119(+));unc-119 (tm4063)) mixed sex mixed stage (embryo) (3 hours) using egg bleaching whole organism genetically modified (insertion) using bombardment targeting eGFP-drap-1ENCBS879FCFENCGM126PQFENCLB956NDA