Experiment summary for ENCSR023VVO
doi:10.17989/ENCSR023VVO
Summary
- Status
- released
- Assay
- polyA plus RNA-seq
- Biosample summary
- Homo sapiens bipolar neuron originated from GM23338 treated with 0.5 μg/mL doxycycline hyclate for 4 days
- Biosample Type
- in vitro differentiated cells
- Replication type
- isogenic
- Description
- PolyA RNA-Seq from oligo-dT primed Total RNA on bipolar spindle neuron
- Treatments
- 0.5 μg/mL doxycycline hyclate (CHEBI:34730) for 4 days
- Nucleic acid type
- polyadenylated mRNA
- Library starting quantity
- 10ng
- Size range
- >200
- Extraction method
- Ambion mirVana
- Fragmentation methods
- chemical (Nextera tagmentation)
- Size selection method
- SPRI beads
- Strand specificity
- unstranded
- Spike-ins datasets
- ENCSR884LPM
- Platform
- Illumina HiSeq 2500
Attribution
ENCODE3 project
- Lab
- Barbara Wold, Caltech
- Award
- U54HG006998 (Richard Myers, HAIB)
- Project
- ENCODE
- External resources
- References
- Date submitted
- April 19, 2016
- Date released
- May 3, 2016
- Reference Epigenome
- ENCSR954HHV
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
|---|---|---|---|---|
| 1 | 1 | Homo sapiens male adult (53 years) bipolar neuron in vitro differentiated cells originated from GM23338 treated with 0.5 μg/mL doxycycline hyclate for 4 days | ENCBS369AAA | ENCLB444LRG |
| 2 | 1 | Homo sapiens male adult (53 years) bipolar neuron in vitro differentiated cells originated from GM23338 treated with 0.5 μg/mL doxycycline hyclate for 4 days | ENCBS374AAA | ENCLB228SAY |