Experiment summary for ENCSR019HJV
doi:10.17989/ENCSR019HJV
Summary
- Status
- released
- Assay
- whole-genome shotgun bisulfite sequencing (WGBS)
- Biosample summary
- Mus musculus strain B6NCrl forebrain tissue embryo (15.5 days)
- Biosample Type
- tissue
- Replication type
- isogenic
- Description
- Whole genome bisulfite-seq on embryonic 15.5 day mouse forebrain
- Nucleic acid type
- DNA
- Size range
- 180-500
- Lysis method
- QIAGEN DNeasy Blood & Tissue Kit
- Fragmentation methods
- shearing (Covaris S2)
- Size selection method
- Sera-mag SpeedBeads
- Platform
- Illumina HiSeq 2500
Attribution
ENCODE3 project
- Lab
- Joe Ecker, Salk
- Award
- U54HG006997 (Bing Ren, UCSD)
- Project
- ENCODE
- External resources
- Aliases
- joe-ecker:E15_5_FB
- Date submitted
- April 29, 2015
- Date released
- June 23, 2015
- Organism Development Series
- ENCSR082AVR
- Reference Epigenome
- ENCSR438IXZ
- Submitter comment
- Fastq files ENCFF964GLF, ENCFF197SOT, ENCFF476SUD, and ENCFF134AUA contain reads of mixed lengths. Read lengths followed by respective number of reads: ENCFF964GLF (109, 204330881), (130, 202153304), (139, 404424458); ENCFF197SOT (109, 205208625), (130, 193365942), (139, 406010415); ENCFF476SUD (130, 195669684), (139, 204965623); ENCFF134AUA (130, 212653076), (139, 205117015).
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Library |
---|---|---|---|---|
1 | 1 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) forebrain tissue | ENCBS811RMD | ENCLB982VJQ |
2 | 2 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) forebrain tissue | ENCBS841GER | ENCLB967VBA |
1 | 2 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) forebrain tissue | ENCBS811RMD | ENCLB813RGF |
2 | 1 | Mus musculus strain B6NCrl mixed sex embryo (15.5 days) forebrain tissue | ENCBS841GER | ENCLB739QRE |