Experiment summary for ENCSR017FMO

Summary

Status
released
Assay
ChIP-seq
Target
eGFP-phol
Biosample summary
Drosophila melanogaster attP40 whole organism embryo (0-16 hours) genetically modified using site-specific recombination
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq on transgenic flies expressing phol-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Size range
200-800
Fragmentation method
sonication (Bioruptor generic)
Size selection method
SPRI
Strand specificity
Non-strand-specific
Controls

Attribution

modERN project
Lab
Kevin White, UChicago
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
Aliases
kevin-white:Phol-GFP
Date released
December 21, 2018

Isogenic replicates

Isogenic replicateTechnical replicateSummaryBiosampleModificationsAntibodyLibrary
21mixed sex embryo (0-16 hours) whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-pholENCBS410IOTENCGM217TYIENCAB728YTOENCLB396BUV
31mixed sex embryo (0-16 hours) whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-pholENCBS289OKTENCGM217TYIENCAB728YTOENCLB405GBY
11mixed sex embryo (0-16 hours) whole organism genetically modified (insertion) using site-specific recombination targeting eGFP-pholENCBS597WJGENCGM217TYIENCAB728YTOENCLB268XBF