Experiment summary for ENCSR010MNU

doi:10.17989/ENCSR010MNU

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
chd-7
Biosample summary
Caenorhabditis elegans strain OP609 (unc-119(tm4063) III; wgIs609) whole organism young adult 67 hours post synchronization at L1 larva starved after bleaching stage genetically modified (insertion) using bombardment targeting C. elegans chd-7
Biosample Type
whole organisms
Replication type
isogenic
Description
ChIP-seq of transgenic worms expressing CHD-7-eGFP fusion proteins. The IP was performed using an anti-GFP antibody.
Nucleic acid type
DNA
Fragmentation methods
sonication (generic microtip)
Size selection method
SPRI beads
Controls

Attribution

modERN project
Lab
Valerie Reinke, Yale
Award
U41HG007355 (Robert Waterston, UW)
Project
modERN
External resources
Aliases
kevin-white:CHD-7-OP609-yAd
Date submitted
October 4, 2016
Date released
October 11, 2016
Submitter comment
ENCFF237EVG, ENCFF632LPW, ENCFF830VRG, ENCFF929JYC were revoked because a software bug accidentally truncated the file content. The submitters will provide replacement files.

Encyclopedia Integration

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
11Caenorhabditis elegans strain OP609 (unc-119(tm4063) III; wgIs609) mixed sex young adult 67 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans chd-7ENCBS637HEXENCGM378DQYENCAB728YTOENCLB398YYT
21Caenorhabditis elegans strain OP609 (unc-119(tm4063) III; wgIs609) mixed sex young adult 67 hours post synchronization at L1 larva starved after bleaching stage whole organism genetically modified (insertion) using bombardment targeting C. elegans chd-7ENCBS782ZZXENCGM378DQYENCAB728YTOENCLB531TQY