Experiment summary for ENCSR010JOI

doi:10.17989/ENCSR010JOI

Summary

Status
released
Assay
RIP-seq
Target
qkr58E-1
Biosample summary
Drosophila melanogaster strain Oregon-R S2R+ stably expressing qkr58E-1 treated with 0.35 mM copper(II) sulfate for 24 hours
Biosample Type
cell line
Replication type
isogenic
Description
RIP-seq targeting qkr58E-1 in Drosophila S2R+ cells.
Treatments
0.35 mM copper(II) sulfate (CHEBI:23414) for 24 hours
Nucleic acid type
RNA
Size range
>200
Fragmentation methods
chemical (generic)
Size selection method
AMPure XP bead purification
Strand specificity
unstranded
Controls

Attribution

modENCODE Project
Lab
Brenton Graveley, UConn
Award
U01HG004271 (Susan Celniker, LBNL)
Project
modENCODE
References
Aliases
modencode:5412, brenton-graveley:S2R+-CG3613-HA_RIP
Date submitted
March 31, 2014
Date released
March 31, 2014

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21Drosophila melanogaster strain Oregon-R S2R+ cell line stably expressing C-terminal FLAG-tagged qkr58E-1 under MtnA promoter, C-terminal HA-tagged qkr58E-1 under MtnA promoter treated with 0.35 mM copper(II) sulfate for 24 hoursENCBS628PBBENCGM960QZSENCAB639FUHENCLB216BEJ
11Drosophila melanogaster strain Oregon-R S2R+ cell line stably expressing C-terminal FLAG-tagged qkr58E-1 under MtnA promoter, C-terminal HA-tagged qkr58E-1 under MtnA promoter treated with 0.35 mM copper(II) sulfate for 24 hoursENCBS510QWAENCGM960QZSENCAB639FUHENCLB791NEB