Experiment summary for ENCSR000DSM

Summary

Status
released
Assay
ChIP-seq (Histone ChIP-seq)
Target
H3K27me3
Biosample summary
Homo sapiens cardiac muscle cell originated from H7 treated with 5 ng/mL Fibroblast growth factor 2 for 14 days, 10 ng/mL Bone morphogenetic protein 4 for 14 days, 6 ng/mL Activin A for 14 days
Biosample Type
in vitro differentiated cells
Replication type
isogenic
Description
H3K27me3 ChIP-seq on human H7-hESC differentiated 14 days
Treatments
5 ng/mL Fibroblast growth factor 2 (UniProtKB:P09038) for 14 days, 10 ng/mL Bone morphogenetic protein 4 (UniProtKB:P12644) for 14 days, 6 ng/mL Activin A (CHEBI:81351) for 14 days
Nucleic acid type
DNA
Lysis method
see document
Extraction method
see document
Fragmentation methods
see document
Size selection method
see document
Strand specificity
Non-strand-specific
Controls

Attribution

ENCODE2 project
Lab
John Stamatoyannopoulos, UW
Award
U54HG004592 (John Stamatoyannopoulos, UW)
Project
ENCODE
Date submitted
June 25, 2011
Date released
February 16, 2012
Related datasets
ENCSR114LRH 
Histone ChIP-seq targeting H3K27me3 on H7-hESCs differentiated into cardiovascular cells over 14 days.
, ENCSR590OZV 
Reference epigenome for human H7-derived cardiac muscle cell
Submitter comment
Replicate 2,1 of this experiment was previously attributed to biosample ENCBS294AAA and replicate 1,1 of this experiment was previously attributed to biosample ENCBS294AAA but they have since been corrected to the current biosamples. Please note that the biosample term has remain unchanged.
Tags
Search for all Experiment with internal tag ENCYCLOPEDIAv5Search for all Experiment with internal tag ENCYCLOPEDIAv4

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Antibody
Library
21female embryo cardiac muscle in vitro differentiated cells originated from H7 treated with 5 ng/mL Fibroblast growth factor 2 for 14 days, 10 ng/mL Bone morphogenetic protein 4 for 14 days, 6 ng/mL Activin A for 14 daysENCBS395GPZENCAB000ANBENCLB728NPN
11female embryo cardiac muscle in vitro differentiated cells originated from H7 treated with 5 ng/mL Fibroblast growth factor 2 for 14 days, 10 ng/mL Bone morphogenetic protein 4 for 14 days, 6 ng/mL Activin A for 14 daysENCBS173KZVENCAB000ANBENCLB767SIL