Experiment summary for ENCSR000DSK
doi:10.17989/ENCSR000DSK
Summary
- Status
- released
- Assay
- ChIP-seq (Histone ChIP-seq)
- Target
- H3K27me3
- Biosample summary
- Homo sapiens cardiovascular progenitor cell originated from H7 treated with 5 ng/mL Fibroblast growth factor 2 for 5 days, 10 ng/mL Bone morphogenetic protein 4 for 5 days, 6 ng/mL Activin A for 5 days
- Biosample Type
- in vitro differentiated cells
- Replication type
- isogenic
- Description
- H3K27me3 ChIP-seq on human H7-hESC differentiated 5 days
- Treatments
- 5 ng/mL Fibroblast growth factor 2 (UniProtKB:P09038) for 5 days, 10 ng/mL Bone morphogenetic protein 4 (UniProtKB:P12644) for 5 days, 6 ng/mL Activin A (CHEBI:81351) for 5 days
- Nucleic acid type
- DNA
- Fragmentation methods
- see document
- Platform
- Illumina Genome Analyzer
- Controls
Attribution
ENCODE2 project
- Lab
- John Stamatoyannopoulos, UW
- Award
- U54HG004592 (John Stamatoyannopoulos, UW)
- Project
- ENCODE
- External resources
- Date submitted
- June 25, 2011
- Date released
- February 16, 2012
- Treatment Time Series
- ENCSR114LRH
- Reference Epigenome
- ENCSR689KKY
- Submitter comment
- Replicate 1,1 of this experiment was previously attributed to biosample ENCBS296AAA and replicate 2,1 of this experiment was previously attributed to biosample ENCBS296AAA but they have since been corrected to the current biosamples. Please note that the biosample term has remain unchanged.
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Antibody | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens female embryo cardiovascular progenitor in vitro differentiated cells originated from H7 treated with 5 ng/mL Fibroblast growth factor 2 for 5 days, 10 ng/mL Bone morphogenetic protein 4 for 5 days, 6 ng/mL Activin A for 5 days | ENCBS360WQQ | ENCAB000ANB | ENCLB322GIT |
| 2 | 1 | Homo sapiens female embryo cardiovascular progenitor in vitro differentiated cells originated from H7 treated with 5 ng/mL Fibroblast growth factor 2 for 5 days, 10 ng/mL Bone morphogenetic protein 4 for 5 days, 6 ng/mL Activin A for 5 days | ENCBS138XIW | ENCAB000ANB | ENCLB112XYM |