Experiment summary for ENCSR000DSI
doi:10.17989/ENCSR000DSI
Summary
- Status
- released
- Assay
- ChIP-seq (Histone ChIP-seq)
- Target
- H3K36me3
- Biosample summary
- Homo sapiens cardiac muscle cell originated from H7 treated with 5 ng/mL Fibroblast growth factor 2 for 14 days, 10 ng/mL Bone morphogenetic protein 4 for 14 days, 6 ng/mL Activin A for 14 days
- Biosample Type
- in vitro differentiated cells
- Replication type
- isogenic
- Description
- H3K36me3 ChIP-seq on human H7-hESC differentiated 14 days
- Treatments
- 5 ng/mL Fibroblast growth factor 2 (UniProtKB:P09038) for 14 days, 10 ng/mL Bone morphogenetic protein 4 (UniProtKB:P12644) for 14 days, 6 ng/mL Activin A (CHEBI:81351) for 14 days
- Nucleic acid type
- DNA
- Fragmentation methods
- see document
- Platform
- Illumina Genome Analyzer
- Controls
Attribution
ENCODE2 project
- Lab
- John Stamatoyannopoulos, UW
- Award
- U54HG004592 (John Stamatoyannopoulos, UW)
- Project
- ENCODE
- External resources
- Date submitted
- June 25, 2011
- Date released
- February 16, 2012
- Treatment Time Series
- ENCSR182ORV
- Reference Epigenome
- ENCSR590OZV
- Submitter comment
- Replicate 1,1 of this experiment was previously attributed to biosample ENCBS294AAA and replicate 2,1 of this experiment was previously attributed to biosample ENCBS294AAA but they have since been corrected to the current biosamples. Please note that the biosample term has remain unchanged.
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Antibody | Library |
|---|---|---|---|---|---|
| 1 | 1 | Homo sapiens female embryo cardiac muscle in vitro differentiated cells originated from H7 treated with 5 ng/mL Fibroblast growth factor 2 for 14 days, 10 ng/mL Bone morphogenetic protein 4 for 14 days, 6 ng/mL Activin A for 14 days | ENCBS395GPZ | ENCAB000AQW | ENCLB046LNX |
| 2 | 1 | Homo sapiens female embryo cardiac muscle in vitro differentiated cells originated from H7 treated with 5 ng/mL Fibroblast growth factor 2 for 14 days, 10 ng/mL Bone morphogenetic protein 4 for 14 days, 6 ng/mL Activin A for 14 days | ENCBS173KZV | ENCAB000AQW | ENCLB624HPW |