Experiment summary for ENCSR000DSF

doi:10.17989/ENCSR000DSF

Summary

Status
released
Assay
ChIP-seq (Histone ChIP-seq)
Target
H3K36me3
Biosample summary
Homo sapiens cardiac myoblast originated from H7 treated with 10 ng/mL Bone morphogenetic protein 4 for 9 days, 5 ng/mL Fibroblast growth factor 2 for 9 days, 6 ng/mL Activin A for 9 days
Biosample Type
in vitro differentiated cells
Replication type
isogenic
Description
H3K36me3 ChIP-seq on human H7-hESC differentiated 9 days
Treatments
10 ng/mL Bone morphogenetic protein 4 (UniProtKB:P12644) for 9 days, 5 ng/mL Fibroblast growth factor 2 (UniProtKB:P09038) for 9 days, 6 ng/mL Activin A (CHEBI:81351) for 9 days
Nucleic acid type
DNA
Fragmentation methods
see document
Controls

Attribution

ENCODE2 project
Lab
John Stamatoyannopoulos, UW
Award
U54HG004592 (John Stamatoyannopoulos, UW)
Project
ENCODE
Date submitted
June 25, 2011
Date released
February 16, 2012
Treatment Time Series
ENCSR182ORV 
Reference Epigenome
ENCSR472YCH 
Submitter comment
Replicate 1,1 of this experiment was previously attributed to biosample ENCBS297AAA and replicate 2,1 of this experiment was previously attributed to biosample ENCBS297AAA but they have since been corrected to the current biosamples. Please note that the biosample term has remain unchanged.
Tags
Search for all Experiment with internal tag ENCYCLOPEDIAv1Search for all Experiment with internal tag ENCYCLOPEDIAv2

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Antibody
Library
11Homo sapiens female embryo cardiac myoblast in vitro differentiated cells originated from H7 treated with 10 ng/mL Bone morphogenetic protein 4 for 9 days, 5 ng/mL Fibroblast growth factor 2 for 9 days, 6 ng/mL Activin A for 9 daysENCBS152EVQENCAB000AQWENCLB138FGU
21Homo sapiens female embryo cardiac myoblast in vitro differentiated cells originated from H7 treated with 10 ng/mL Bone morphogenetic protein 4 for 9 days, 5 ng/mL Fibroblast growth factor 2 for 9 days, 6 ng/mL Activin A for 9 daysENCBS303BHKENCAB000AQWENCLB082UHX