1. Experiments
  2. ChIP-seq
  3. Homo sapiens
  4. MCF 10A

Experiment summary for ENCSR000DOP

Summary

Status
released
Assay
ChIP-seq (TF ChIP-seq)
Target
FOS
Biosample summary
Homo sapiens MCF 10A genetically modified using stable transfection treated with 1 μM afimoxifene for 12 hours
Biosample Type
cell line
Replication type
isogenic
Description
FOS ChIP-seq on human MCF10A-Er-Src treated with 4-hydroxy-tamoxifen at 1uM for 12 hours
Treatments
1 μM afimoxifene (CHEBI:44616) for 12 hours
Nucleic acid type
DNA
Lysis method
see document
Extraction method
see document
Fragmentation methods
see document
Size selection method
see document
Strand specificity
Non-strand-specific
Controls

Attribution

ENCODE2 project
Lab
Kevin Struhl, HMS
Award
U54HG004558 (Michael Snyder, Stanford)
Project
ENCODE
Date submitted
October 20, 2010
Date released
May 14, 2012
Related datasets
ENCSR568RJK 
1 μM afimoxifene treatment time series using ChIP-seq of MCF 10A; target: FOS
Tags
Search for all Experiment with internal tag ENCYCLOPEDIAv5Search for all Experiment with internal tag ENCYCLOPEDIAv4

Isogenic replicates

Isogenic replicate
Technical replicate
Summary
Biosample
Modifications
Antibody
Library
21MCF 10A cell line stably expressing SRC treated with 1 μM afimoxifene for 12 hoursENCBS620ENCENCGM980FIGENCAB000AEQENCLB119JWD
11MCF 10A cell line stably expressing SRC treated with 1 μM afimoxifene for 12 hoursENCBS620ENCENCGM980FIGENCAB000AEQENCLB119JWE