Experiment summary for ENCSR000DOP
Summary
- Status
- released
- Assay
- ChIP-seq (TF ChIP-seq)
- Target
- FOS
- Biosample summary
- Homo sapiens MCF 10A genetically modified using stable transfection treated with 1 μM afimoxifene for 12 hours
- Biosample Type
- cell line
- Replication type
- isogenic
- Description
- FOS ChIP-seq on human MCF10A-Er-Src treated with 4-hydroxy-tamoxifen at 1uM for 12 hours
- Treatments
- 1 μM afimoxifene (CHEBI:44616) for 12 hours
- Nucleic acid type
- DNA
- Fragmentation methods
- see document
- Platform
- Illumina Genome Analyzer
- Controls
Attribution
ENCODE2 project
- Lab
- Kevin Struhl, HMS
- Award
- U54HG004558 (Michael Snyder, Stanford)
- Project
- ENCODE
- External resources
- Date submitted
- October 20, 2010
- Date released
- May 14, 2012
- Treatment Time Series
- ENCSR568RJK 1 μM afimoxifene treatment time series using ChIP-seq of MCF 10A; target: FOS
- Tags
Isogenic replicates
Isogenic replicate | Technical replicate | Summary | Biosample | Modifications | Antibody | Library |
|---|---|---|---|---|---|---|
| 2 | 1 | MCF 10A cell line stably expressing SRC treated with 1 μM afimoxifene for 12 hours | ENCBS620ENC | ENCGM980FIG | ENCAB000AEQ | ENCLB119JWD |
| 1 | 1 | MCF 10A cell line stably expressing SRC treated with 1 μM afimoxifene for 12 hours | ENCBS620ENC | ENCGM980FIG | ENCAB000AEQ | ENCLB119JWE |